Ouabain-Binding Protein(s) From Human Plasma
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چکیده
منابع مشابه
Ouabain-binding protein(s) from human plasma.
Conservation of the binding site on mammalian Na+,K+-ATPase for cardiac glycosides and the importance of the Na+ pump in mammalian cellular physiology has stimulated the search for a mammalian analog of these plant compounds. One candidate, isolated from brain and blood, appears to be ouabain itself or a closely related isomer, the ouabain-like compound. Little is known about the circulating fo...
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An enzyme-linked immunosorbent assay is described for the measurement of ouabain in human plasma. This assay is specific for ouabain, strophanthidin, and ouabagenin, with other steroids, including digoxin and vasopressor hormones, exhibiting negligible cross-reactivity. Assay sensitivity was 0.06 nmol/L if 1 mL plasma was extracted and less than 0.005 nmol/L when 20 mL plasma was analyzed. Extr...
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The conditions necessary for optimal ouabain binding in the avian salt gland were examined. Binding was enhanced by ATP and Mg2+ and was decreased by K+, but was unaffected by added Na+. Both maximal binding and complete inhibition of Na, K-ATPase activity were obtained at 1 X 10(-6) M ouabain. Half maximal binding and half maximal inhibition of Na, K-ATPase activity were obtained at 1.7 X 10(-...
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ژورنال
عنوان ژورنال: Hypertension
سال: 2002
ISSN: 0194-911X,1524-4563
DOI: 10.1161/01.hyp.0000027134.14160.1d